china a23483 cleaved parp1 mouse (Proteintech)

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Proteintech china a23483 cleaved parp1 mouse
China A23483 Cleaved Parp1 Mouse, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cleaved+parp1/PARP1+Fusion+Protein/pm41713621-94-106-112
Average 95 stars, based on 39 article reviews

china a23483 cleaved parp1 mouse - by Bioz Stars, 2026-09

95/100 stars

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Incubation:

Article Title: An anoikis-related signature predicts prognosis and immunotherapy response in gastrointestinal cancers
Article Snippet: The protein samples were electrophoresed on sodium dodecylsulfate−polyacrylamide gels and transferred to polyvinylidene fluoride membranes (Millipore, MA, USA). .. The membranes were blocked with 5% milk and then incubated with primary antibodies against S100A11 (1:1000, Proteintech, 10237-1-AP, China), TLR3 (1:1000, ABclonal, A11778, China), β-actin (1:2000, HUABIO, EM21002, China), BAX (1:20000, HUABIO, ET1702-53, China), Bcl-2 (1:2000, HUABIO, ET1702-53, China), Caspase-3 (1:2000, HUABIO, ET1602-39, China), active Caspase-3 (1:1000, HUABIO, ET1602-47, China), Caspase-7 (1:1000, HUABIO, JE59-36, China), active Caspase-7 (1:1000, HUABIO, ER60002, China), PARP1 (1:10000, Proteintech, 66520-1-Ig, China), and cleaved PARP1 (1:10000, Proteintech, 60555-1-Ig, China) overnight at 4°C. ..

Article Title: Polystyrene particles induces asthma-like Th2-mediated lung injury through IL-33 secretion
Article Snippet: .. The proteins were transferred to polyvinylidene difluoride membranes (Merck Millipore) at 250 mA for 60 min. Non-specific sites were blocked using 5% non-fat dry milk in Tris-buffered saline/Tween 20 (TBS-T) for 1 h. The membrane was incubated with primary antibodies against IFNγ (Santa Cruz, sc-57208), IL-4 (R&D system, MAB404), IL-5 (Santa Cruz, sc-398334), IL-13 (Santa Cruz, sc-393365), IL-17 (Abcam, ab79056), IL-25 (Invitrogen, PA5-100835), IL-33 (Invitrogen, MA5-15773), IL-33 (R&D system, AF3626), poly(ADP-ribose) polymerase 1 (PARP1; Abcam, ab227244), cleaved PARP1 (Proteintech, 13371-1-AP), caspase 3 (R&D system, MAB835), cleavedcaspase 3 (NOVUS, NB100-56708SS), p-IκB-α (Cell Signaling, 9246S), IκB-α (Cell Signaling, 4812S), p-NF-κB (Cell Signaling, 3033S), NF-κB (Cell Signaling, 8242S), and β-actin (Santa Cruz, sc-47778) overnight at 4°C. .. After washing thrice with TBS-T, the membrane was incubated with antirabbit (Cell Signaling, 7074S), anti-mouse (Cell Signaling, 7076S), anti-goat (Santa Cruz, sc-2354), and anti-rat (Cell Signaling, 7077S) antibodies for 1 h at RT.

Article Title: An anoikis-related signature predicts prognosis and immunotherapy response in gastrointestinal cancers.
Article Snippet: The protein samples were electrophoresed on sodium dodecylsulfate −polyacrylamide gels and transferred to polyvinylidene fluoride membranes (Millipore, MA, USA). .. The membranes were blocked with 5% milk and then incubated with primary antibodies against S100A11 (1:1000, Proteintech, 10237-1-AP, China), TLR3 (1:1000, ABclonal, A11778, China), b-actin (1:2000, HUABIO, EM21002, China), BAX (1:20000, HUABIO, ET1702-53, China), Bcl-2 (1:2000, HUABIO, ET1702-53, China), Caspase-3 (1:2000, HUABIO, ET1602-39, China), active Caspase-3 (1:1000, HUABIO, frontiersin.org ET1602-47, China), Caspase-7 (1:1000, HUABIO, JE59-36, China), active Caspase-7 (1:1000, HUABIO, ER60002, China), PARP1 (1:10000, Proteintech, 66520-1-Ig, China), and cleaved PARP1 (1:10000, Proteintech, 60555-1-Ig, China) overnight at 4°C. ..

Saline:

Membrane:

Staining:

Article Title: Ganjie Decoction protects against respiratory syncytial virus infection by activating PI3K/AKT-apoptosis axis and regulating gut microbiota metabolism.
Article Snippet: Ethnopharmacological relevance: Ganjie Decoction (GJD), a traditional Chinese medicine (TCM) formula commonly used for respiratory diseases, has shown therapeutic potential against RSV pneumonia.. However, its pharmacological mechanisms against respiratory syncytial virus (RSV) pneumonia are not fully understood.. Aim of study: This study aimd to characterize the active components of GJD and systematically investigate its therapeutic effects and underlying mechanisms in RSV-induced pneumonia.

Immunostaining:

Western Blot:

Article Title: Bioinformatics analysis combined with experimental validation reveals the novel mechanisms of multi-targets of dapagliflozin attenuating diabetic liver injury
Article Snippet: The concentrations of GSH (Cat# A005-1-2), MDA (Cat#A003-1-2), CAT (Cat#A007-1-1),and SOD (Cat#A001-3-2), AST (C010-2-1), ALT (C009-2-1), TG (A110-1-1), T-CHO (A111-1-1) were purchased from Nanjing Jiancheng Bioengineering Institute, Nanjing, China. .. The primary antibodies including p-ERK1/2 (CST, Cat#4370S, 1:1000), ERK1/2 (CST, Cat#9120S, 1:1000), IKKβ (Wanleibio, Cat#WL04340, 1:1000), Cleaved PARP1 (Proteintech, Cat#60555-1-Ig, 1:1000), TNF-α (ABclonal, Cat#A11534, 1:1000), p-NF-κB p65 (Abcam, Cat#ab86299, 1:1000), NF-κB p65 (Bioworld, Cat#bs1253 1:1000), SGLT2 (Biodragon, Cat#BD-PT4274, 1:1000) BAX (ABclonal, Cat#A15646, 1:1000), BCL2 (Bioss, Cat#bs-0032R, 1:1000), Cleaved-caspase3 (CST, Cat#9661S, 1:1000), Caspase3 (CST, Cat#9662, 1:1000), IL-6 (ABclonal, Cat#A0286, 1:1000), IL-18 (ABclonal, Cat#A20473, 1:1000), and IL-1β (SAB, Cat#41059-2, 1:1000), GAPDH (Cat#LF205; Epizyme, shanghai, 1:5000), β-actin (Cat#LF201; Epizyme, shanghai, 1:10000), α-tubulin (Cat#LF210; Epizyme, shanghai, 1:10000) were used for western blotting. ..

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